Table 3

PCR sensitivities for the detection of seven different HPV types. HPV recombinant plasmids were diluted in a background of 100 ng C-33A DNA and amplified (50 cycles) by the GP5+/GP6+ protocol or by the TDP3 method. HPV detected, HPV undetected, Σm Total number of mismatches between the GP5+/GP6+ primers and the target HPV type.

GP5+/GP6+

TDP3


Σm

HPV

1.5pg

150fg

15fg

1.5fg

150ag

15ag

1.5pg

150fg

15fg

1.5fg

150ag

15ag


2

16

3

33

3

45

4

31

4

56

7

52

10

51


Evans et al. BMC Clinical Pathology 2005 5:10   doi:10.1186/1472-6890-5-10

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