Figure 5.

Dot blot hybridization detection of multiple HPV types after TDP3 PCR. HPV types 16, 45, 51, and 52 were combined (1.5pg, 150fg, 15fg, 1.5fg, or 150ag of each type) in a background of 100 ng C-33A DNA. After PCR, 0.6 μl of denatured product was spotted onto nylon membrane and hybridized with biotin-labeled HPV type specific oligonucleotide probe. Duplicate dot preparations were prepared.

Evans et al. BMC Clinical Pathology 2005 5:10   doi:10.1186/1472-6890-5-10
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