Dot blot hybridization detection of multiple HPV types after TDP3 PCR. HPV types 16, 45, 51, and 52 were combined (1.5pg, 150fg, 15fg, 1.5fg, or 150ag of each type) in a background of 100 ng C-33A DNA. After PCR, 0.6 μl of denatured product was spotted onto nylon membrane and hybridized with biotin-labeled HPV type specific oligonucleotide probe. Duplicate dot preparations were prepared.
Evans et al. BMC Clinical Pathology 2005 5:10 doi:10.1186/1472-6890-5-10