Detection of HPV-16 PCR product by agarose gel electrophoresis. SiHa cell DNA, 0, 0.1, 0.5, 1.0, 25, or 100 ng, was diluted in C-33A DNA to give a total of 0.5 μg or 0.1 μg DNA. The GP5+/GP6+, TDP1, TDP2, TDP3 and TDP4 assays were compared for the detection of HPV-16 product on an ethidium bromide (0.5 μg/ml) stained 2.5% agarose gel. RB: reagent blank, M: 50 base pair molecular weight ladder.
Evans et al. BMC Clinical Pathology 2005 5:10 doi:10.1186/1472-6890-5-10