Specificity of immunohistochemical detection of the PI3K-C2 enzymes using antibodies raised against class II N-terminal peptide fragments. Lysates from breast (MCF-7 and T47D, lanes 1 and 2) and prostate (CWR22Rv1, LNCaP, PC3 and DU145 cells, lanes 3–6 respectively) cancer cell lines were probed for PI3K antibodies (p85, PI3K-C2α and PI3K-C2β). Molecular weight markers in KDa are indicated on the left. In the lower panels, representative PI3K-C2α and PI3K-C2β labelled sections with (B and D respectively) or without pre-absorption of each antibody with its antigenic peptide (0.1mg/ml of N-terminal PI3K-C2α in C and 0.5mg/ml of N-terminal PI3K-C2β in E) are presented. Original magnification (A-D × 200).
El Sheikh et al. BMC Clinical Pathology 2003 3:4 doi:10.1186/1472-6890-3-4