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Open Access Research article

P53 nuclear stabilization is associated with FHIT loss and younger age of onset in squamous cell carcinoma of oral tongue

Raju SR Adduri1, Viswakalyan Kotapalli1, Neha A Gupta16, Swarnalata Gowrishankar2, Mukta Srinivasulu3, Mohammed Mujtaba Ali3, Subramanyeshwar Rao37, Shantveer G Uppin4, Umanath K Nayak2, Snehalatha Dhagam5, Mohana Vamsy Chigurupati5 and Murali Dharan Bashyam1*

Author Affiliations

1 Laboratory of Molecular Oncology, Centre for DNA Fingerprinting and Diagnostics, Nampally, Hyderabad 500001, India

2 Apollo Hospitals, Jubilee Hills, Hyderabad India

3 MNJ Institute of Oncology & Regional Cancer Centre, Red Hills, Hyderabad India

4 Nizam’s Institute of Medical Sciences, Punjagutta, Hyderabad India

5 Omega Hospitals, Jubilee Hills, Hyderabad, India

6 Currently at National Centre for Cell Science, Ganeshkhind, Pune, India

7 Currently at Basavatarakam Indo American Cancer Hospital & Research Institute, Hyderabad, India

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BMC Clinical Pathology 2014, 14:37  doi:10.1186/1472-6890-14-37

Published: 9 August 2014

Abstract

Background

Squamous cell carcinoma of tongue (SCCT) is expected to harbor unique clinico-pathological and molecular genetic features since a significant proportion of patients are young and exhibit no association with tobacco or alcohol.

Methods

We determined P53, epidermal growth factor receptor, microsatellite instability, human papilloma virus infection and loss of heterozygosity status at several tumor suppressor loci in one hundred and twenty one oral SCCT (SSCOT) samples and analyzed their association with clinico-pathological features and patient survival.

Results

Our results revealed a significantly higher incidence of p53 nuclear stabilization in early (as against late) onset SCCOT. FHIT loss was significantly associated with p53 nuclear stabilization and the association was stronger in patients with no history of tobacco use. Samples harboring mutation in p53 DNA binding domain or exhibiting p53 nuclear stabilization, were significantly associated with poor survival.

Conclusion

Our study has therefore identified distinct features in SCCOT tumorigenesis with respect to age and tobacco exposure and revealed possible prognostic utility of p53.

Keywords:
Oral tongue cancer; TP53; FHIT; EGFR; Disease specific survival