Open Access Research article

Evaluation of a solid matrix for collection and ambient storage of RNA from whole blood

Heng Tao1, Philip Beineke1, Bing Li2, William Alberts2, Steven Rosenberg1, Erik Kvam2* and James A Wingrove1*

Author Affiliations

1 CardioDx, Inc., 2500 Faber Place, Palo Alto, CA 94303, USA

2 GE Global Research, One Research Circle, K1 5D29 Niskayuna NY 12309, USA

For all author emails, please log on.

BMC Clinical Pathology 2014, 14:22  doi:10.1186/1472-6890-14-22

Published: 13 May 2014

Additional files

Additional file 1: Table S1:

MIQE data for the 23 genes used to generate the GES.

Format: XLSX Size: 47KB Download file

Open Data

Additional file 2: Figure S1:

Experimental flow for 25 subjects used in the PAXgene vs. RSM comparison (A) and for the 10 PAXgene pool control samples (B).

Format: PDF Size: 98KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 3: Figure S2:

Agilent Bioanalyzer traces of the RNA isolated from the 25 subjects collected in PAXgene tubes and stored overnight at RT (A); 25 subjects collected on RSM paper and stored overnight at RT (B); 10 subjects collected on 31-ETF paper and stored overnight at RT (C); 10 subjects collected on RSM and stored overnight at 37°C (D); 10 subjects collected on RSM and stored for 6 days at RT (E); 10 PAXgene pool controls stored overnight at RT (F); 10 PAXgene pool controls stored overnight at 37°C (G); 10 PAXgene pool controls stored for 6 days at RT.

Format: PDF Size: 1.2MB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data