Additional file 3.
Screening of SULT1A1 gene expression activators from MCF-7 cells treated with siRNAs. SULT1A1 mRNA levels were determined with The Expressed Transcription Factor Knockdown Transcriptome PCR Array (Qiagen, Valencia, CA) following the manufacturer’s instructions. Briefly, real-time PCR was performed with cDNAs derived from MCF-7 cells treated with siRNAs targeting 270 transcription factors. SULT1A1 gene expression levels were expressed as Log2 fold changes based on Ct calculation using 18S as house-keeping gene and non-target siRNA treated sample well (VTC) as negative control. The arrow indicates SULT1A1 gene expression was decreased more than four-fold in siNFI-B transfected MCF-7 cells.
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Yao-Borengasser et al. BMC Clinical Pathology 2014 14:1 doi:10.1186/1472-6890-14-1