Open Access Research article

Sulfotransferase 1A1 (SULT1A1) gene expression is regulated by members of the NFI transcription factors in human breast cancer cells

Aiwei Yao-Borengasser, Lora J Rogers, Vineetha K Edavana, Rosalind B Penney, Xinfeng Yu, Ishwori B Dhakal, Suzanne Williams and Susan A Kadlubar*

Author Affiliations

Division of Medical Genetics, College of Medicine, University of Arkansas for Medical Sciences, 4301 West Markham St., Little Rock, AR, 72205, USA

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BMC Clinical Pathology 2014, 14:1  doi:10.1186/1472-6890-14-1

Published: 6 January 2014

Additional files

Additional file 1:

Differential transcription factor activities levels between MCF-10A and ZR-75-1 cells. Transcription factor activation profile was determined using a TF Activation Profiling Plate Array as described in Methods. Data chosen from results showing that transcription factor activation level in ZR-75-1 cells (black bar) was at least 1.5 fold higher than that in MCF-10A cells (gray bar).

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Additional file 2:

Correlation between GATA-1 mRNA and NFI-C mRNA in Human liver. Real-time RT-PCR was performed in liver samples taken from healthy subjects as described in Methods. Data was normalized with 18S. GATA-1 mRNA level was not correlated with SULT1A1 mRNA level (r = 0.17, p = 0.11).

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Additional file 3:

Screening of SULT1A1 gene expression activators from MCF-7 cells treated with siRNAs. SULT1A1 mRNA levels were determined with The Expressed Transcription Factor Knockdown Transcriptome PCR Array (Qiagen, Valencia, CA) following the manufacturer’s instructions. Briefly, real-time PCR was performed with cDNAs derived from MCF-7 cells treated with siRNAs targeting 270 transcription factors. SULT1A1 gene expression levels were expressed as Log2 fold changes based on Ct calculation using 18S as house-keeping gene and non-target siRNA treated sample well (VTC) as negative control. The arrow indicates SULT1A1 gene expression was decreased more than four-fold in siNFI-B transfected MCF-7 cells.

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