Protein expression in glioblastoma cell lines. VRK1, VRK2, EGFR and cyclin D1 protein levels were determined in three glioblastoma cell lines (A). At the bottom the ratio of EGFR expression respect to VRK proteins expression in each cell line is shown. Growth rate of astrocytoma cell lines (B). Cells were seeded in P60 dishes and were serum deprived for 24 hours. At time 0, serum was added and cell number was determined at different time points after serum readdition. To the right is shown the growth curve of each cell line at different time points after serum readdition. The values are the mean of three independent experiments and the standard deviations are shown. *** P < 0.001.
Rodríguez-Hernández et al. BMC Clinical Pathology 2013 13:23 doi:10.1186/1472-6890-13-23