Double detection of CTHRC1 and NFE2L3 by DELFIA. A: Detection of recombinant CTHRC1 and a non-related biomarker as negative control protein. B: Detection of recombinant NFE2L3 and a non-related biomarker as negative control protein. The sFab E5 and MAb CH21D7 were immobilised in DELFIA yellow plate. The plates were blocked with blocking solution. A mixture of recombinant CTHRC1 and NFE2L3 was added to the wells. The captured antigens were detected with rabbit PAb α-NFE2L3 and biotin-conjugated CH24D7, followed by Europium-labelled anti rabbit antibodies and Samarium-labelled streptavidin. The signal was amplified by enhancement solution. The amount of antibodies was detected by dissociation-enhanced time-resolved fluoroimmunoassays research fluorometer.
Palma et al. BMC Clinical Pathology 2012 12:2 doi:10.1186/1472-6890-12-2