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Open Access Research article

Immunohistochemical detection of laminin-1 and Ki-67 in radicular cysts and keratocystic odontogenic tumors

Mohamed S Ayoub1*, Houry M Baghdadi2 and Moataz El-Kholy3

Author affiliations

1 Professor, Oral Pathology Department, Faculty of Dentistry, Ain Shams University, Cairo, Egypt

2 Associate Professor, Oral Pathology Department, Faculty of Dentistry, Ain Shams University, Cairo, Egypt

3 Assistant Lecturer, Oral Pathology Department, Faculty of Dental Surgery, Modern Science and Arts University, Cairo, Egypt

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Citation and License

BMC Clinical Pathology 2011, 11:4  doi:10.1186/1472-6890-11-4

Published: 2 March 2011

Abstract

Background

Odontogenic cysts are those which arise from the epithelium associated with the development of teeth. Some odontogenic cysts were found to have special biological features that make them distinct from other lesions. This study was conducted to detect the immunoepxression of laminin-1 and Ki-67 in both radicular cysts (RCs) and keratocystic odontogenic tumors (KCOTs) and to examine the possible predictive value of these markers.

Methods

Thirteen cases of RCs and twelve cases of KCOTs were included in this study. Antibodies against laminin-1 and Ki-67 were used as primary antibodies.

Results

ten cases out of thirteen cases of RCs were immunopositive to laminin-1. The immunonegative cases of RCs showed high degree of inflammation inside the connective tissue wall. One case out of twelve cases of KCOTs was immunopositive to laminin-1 and the rest were immunonegative. Seven cases out of thirteen cases of RCs showed immunopositivity for Ki-67 with increased numbers of immunopositive cells when the inflammation was severe in the connective tissue wall. All KCOTS were immunopositive to Ki-67.

Conclusions

The benign nature of radicular cysts and the aggressive behavior of keratocystic odontogenic tumors could be explained by the expression of laminin and Ki-67. Laminin-1 and Ki-67 could be valuable markers for the prediction of the biologic behavior of cystic lesions.