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Open Access Research article

Decrease in thyroid adenoma associated (THADA) expression is a marker of dedifferentiation of thyroid tissue

Lars Kloth1, Gazanfer Belge1, Käte Burchardt2, Siegfried Loeschke1, Werner Wosniok3, Xin Fu1, Rolf Nimzyk1, Salah A Mohamed4, Norbert Drieschner1, Volkhard Rippe1 and Jörn Bullerdiek1*

Author Affiliations

1 Center for Human Genetics, University of Bremen, Leobener Str. ZHG, 28359 Bremen, Germany

2 Department of Pathology, Clinical Center Bremen-Mitte, St. Jürgen Str. 1, 28177 Bremen, Germany

3 Institute of Statistics, University of Bremen, Achterstr. 30, 28359 Bremen, Germany

4 Department of Cardiac Surgery, University Medical Center Schleswig-Holstein, Campus Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany

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BMC Clinical Pathology 2011, 11:13  doi:10.1186/1472-6890-11-13

Published: 4 November 2011

Abstract

Background

Thyroid adenoma associated (THADA) has been identified as the target gene affected by chromosome 2p21 translocations in thyroid adenomas, but the role of THADA in the thyroid is still elusive. The aim of this study was to quantify THADA gene expression in normal tissues and in thyroid hyper- and neoplasias, using real-time PCR.

Methods

For the analysis THADA and 18S rRNA gene expression assays were performed on 34 normal tissue samples, including thyroid, salivary gland, heart, endometrium, myometrium, lung, blood, and adipose tissue as well as on 85 thyroid hyper- and neoplasias, including three adenomas with a 2p21 translocation. In addition, NIS (sodium-iodide symporter) gene expression was measured on 34 of the pathological thyroid samples.

Results

Results illustrated that THADA expression in normal thyroid tissue was significantly higher (p < 0.0001, exact Wilcoxon test) than in the other tissues. Significant differences were also found between non-malignant pathological thyroid samples (goiters and adenomas) and malignant tumors (p < 0.001, Wilcoxon test, t approximation), anaplastic carcinomas (ATCs) and all other samples and also between ATCs and all other malignant tumors (p < 0.05, Wilcoxon test, t approximation). Furthermore, in thyroid tumors THADA mRNA expression was found to be inversely correlated with HMGA2 mRNA. HMGA2 expression was recently identified as a marker revealing malignant transformation of thyroid follicular tumors. A correlation between THADA and NIS has also been found in thyroid normal tissue and malignant tumors.

Conclusions

The results suggest THADA being a marker of dedifferentiation of thyroid tissue.