Additional file 2.
SDS gel of MBD purification. The Coomassie stained SDS gel outlines our simplified MBD purification protocol and enabled us to control correct MBD-bead assembly. Aliquots of the several purification steps were loaded onto the gel. Lane 1, 5 kDa Page Ruler; Lane 2, crude lysate (1:5 dilution); Lane 3, supernatant of the binding reaction; Lane 4 and 5, subsequent washing steps with buffer A containing 10 mM imidazol; Lane 6, purified MBD protein eluted from Ni-NTA beads, where one band at 11 kDa remained.
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Wielscher et al. BMC Clinical Pathology 2011 11:11 doi:10.1186/1472-6890-11-11