Additional file 1.
Differential methylated DNA fragments. Ct-values of differential methylated DNA fragments: DNA was isolated from serum (source 1, n = 8) either using the silica membrane based approach or the MBD approach. Four different 5'-UTR regions with known methylation status in healthy peripheral blood were tested, SALL3, ESR1, CHFR (all unmethylated loci) and ZNF502 (biallelic methylated loci). A mean difference between Ct-values regarding the three unmethylated genes of 2.2 was observed; highlighting the reduced amounts of unmethylated DNA, isolated by MBD loaded bead approach. For the ZNF502 gene locus, which is methylated in healthy adults, no significant difference between Ct-values of two isolation approaches was detected.
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Wielscher et al. BMC Clinical Pathology 2011 11:11 doi:10.1186/1472-6890-11-11