Table 3

COLD-PCR Enhancement According to Specific KRAS Mutation

Mutation

Base Change

Frequency in Colorectal Cancer

Predicted To Enhance With Fast COLD-PCR?

Fold-Enhancement With COLD-PCR


G12D

G

    G
T to G
    A
T

35%

Yes

11.5

G12V

G

    G
T to G
    T
T

22%

Yes

11.5

G13D

G

    G
C to G
    A
C

19%

Yes

9.8

G12C

    G
GT to
    T
GT

9%

Yes

14.0

G12S

    G
GT to
    A
GT

7%

Yes

ND

G12A

G

    G
T to G
    C
T

7%

No

ND

G12R

    G
GT to
    C
GT

1%

No

1.1

G13R

    G
GC to
    C
GC

< 1%

No

2.5

Other

Multiple

< 1%

Yes/No

ND


ND = no data; Frequency Is Based on KRAS Mutations Colorectal Cancer Reported in the COSMIC Database accessed July 2010 http://www.sanger.ac.uk/perl/genetics/CGP webcite.

Pritchard et al. BMC Clinical Pathology 2010 10:6   doi:10.1186/1472-6890-10-6

Open Data