Table 2

Fast COLD-PCR Cycling Program

Step

Temp

Time

Analysis Mode

Acquisition Mode


UNG

40°C

10 m

None

None

Denaturation

94°C

10 m

None

None

Regular-PCR (10 cycles)

94°C

1 s

None

None

52°C

20 s

None

None

72°C

10 s

None

None

COLD-PCR (40 cycles)

81 °C (Tc)

1 s

None

None

Ramp 2.0°C/s

52°C

20 s

Quant

Single

72°C

10 s

None

None

Melting curve

94°C

5 s

None

None

45°C

30 s

None

None

80°C

Ramp 0.15°C/s

Melting

Continuous

Cooling

40°C

10 s

None

None


UNG: Uracil-N-Glycosylase; Temp: Temperature.

Pritchard et al. BMC Clinical Pathology 2010 10:6   doi:10.1186/1472-6890-10-6

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