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COLD-PCR enhanced melting curve analysis improves diagnostic accuracy for KRAS mutations in colorectal carcinoma

Colin C Pritchard1*, Laura Akagi1, Poluru L Reddy2, Loren Joseph2 and Jonathan F Tait1

Author Affiliations

1 Department of Laboratory Medicine, University of Washington, Seattle, USA

2 Department of Pathology, University of Chicago, Chicago, USA

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BMC Clinical Pathology 2010, 10:6  doi:10.1186/1472-6890-10-6

Published: 26 November 2010

Additional files

Additional file 1:

Supplemental Figures S1-S3. Supplemental figures S1, S2, and S3, with figure legends.

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Additional file 2:

Table S1. Supplemental Table 1: Complete list of mutations detected by regular- or COLD-PCR.

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Additional file 3:

Sequencing results on the re-classified specimens. Regular- and COLD-PCR sequencing data for the 4 re-classified (discrepant) specimens.

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