Additional file 1.
Controls and antibody specificity. A, SiHa and ME180 xenografts grown in SCID mice show mesenchymal and squamous characteristics, respectively. Sections of both xenografts were used as positive and negative controls. B, Placenta, SiHa xenograft and spindle cell carcinoma (SpCC) of the head and neck were used to test SNAI1 antibody specificity in a competition assay. Absence of staining after incubation with SC10432P blocking peptide confirms specific staining in cell nuclei. C, SiRNA treatment of SiHa cells confirms antibody specificity in an immunoblot performed with AF3639 on SiHa monolayer lysates. D, Comparison of AF3639 and SC10432 shows identical nuclear staining of the extravillous trophoblast. IHC with SC10432 additionally results in some focal cytoplasmic staining (asterisk) which can also be seen inside the villi in section B of this figure.
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Schwock et al. BMC Clinical Pathology 2010 10:1 doi:10.1186/1472-6890-10-1