Figure 2.

A) Western blot of lysates from whole placenta (WP), trophoblast culture (TrC), rectus fascia (RF), and levator ani muscle of the symptomatic (sLM) and asymptomatic woman (aLM). Note a single band detected by p27 monoclonal antibody (lanes 1–5) and a lack of nonspecific staining (lanes 6 and 7). B) Quantitative evaluation of panel A showed significant differences in the density of p27 expression between all measurements (P < 0.001), but not WP vs. RF (P > 0.05) and sLM vs. aLM (P > 0.05).