Morphological changes of bladder carcinoma J82 and bladder urothelial UROtsa cells following frankincense oil stimulation. Bladder J82 and UROtsa cells were seeded in 96-well tissue culture plates at the concentration of 1 × 104 cells/mm2, cultured overnight for adherence, and either left untreated or subjected 1:1,000 dilution of frankincense oil stimulation. Images were taken at 24 hours following treatments for (A) untreated J82 cells, (B) J82 cells treated with frankincense oil, (C) untreated UROtsa cells, and (D) UROtsa cells treated with frankincense oil using Olympus IX51 inverted microscope. Notice cell shrinkage observed in J82 cells following frankincense oil treatment. In contrast, UROtsa cells did not experience noticeable morphological alteration following the same concentration of frankincense oil exposure.
Frank et al. BMC Complementary and Alternative Medicine 2009 9:6 doi:10.1186/1472-6882-9-6