Figure 6.

TLC silica fractionation of fraction AB-P1 and triterpenoid detection. Lane 1 (DHT) = 20 μl of 1 mM dihydrotestosterone spotted on, band-wise. Lane 2 (Aucubin): 20 μl of 10 uM aucubin spotted on, band-wise. Lane 3 (AB-P1): 20 μl of fraction AB-P1 (1c.f.) spotted on, band-wise. Colorimetric development was carried out using vanillin-sulphuric acid reaction to detect presence of triterpenoids.