Email updates

Keep up to date with the latest news and content from BMC Complementary and Alternative Medicine and BioMed Central.

Open Access Research article

Cytisus scoparius link - A natural antioxidant

Raja Sundararajan1, Nazeer Ahamed Haja1, Kumar Venkatesan1, Kakali Mukherjee1, Bishnu Pada Saha1, Arun Bandyopadhyay2 and Pulok Kumar Mukherjee1*

Author Affiliations

1 School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700 032, India

2 Molecular Endocrinology Laboratory, Indian Institute of Chemical Biology, Kolkata 700 032, India

For all author emails, please log on.

BMC Complementary and Alternative Medicine 2006, 6:8  doi:10.1186/1472-6882-6-8

Published: 16 March 2006

Abstract

Background

Recent investigations have shown that the antioxidant properties of plants could be correlated with oxidative stress defense and different human diseases. In this respect flavonoids and other polyphenolic compounds have gained the greatest attention. The plant Cytisus scoparius contains the main constituent of flavone and flavonals. The present study was undertaken to evaluate the in vitro antioxidant activities of extract of aerial part of Cytisus scoparius.

Methods

The plant extract was tested for DPPH (1, 1-diphenyl, 2-picryl hydrazyl) radical scavenging, nitric oxide radical scavenging, superoxide anion radical scavenging, hydroxyl radical scavenging, antilipid peroxidation assay, reducing power and total phenol content.

Results

The extract exhibited scavenging potential with IC50 value of 1.5 μg/ml, 116.0 μg/ml and 4.7 μg/ml for DPPH, nitric oxide and superoxide anion radicals. The values were found to lesser than those of vitamin C, rutin, and curcumin, as standards. The extract showed 50% protection at the dose of 104.0 μg/ml in lipid peroxidation induced by Fe2+/ ascorbate system in rat liver microsomal preparation. There is decrease in hydroxyl radical generation with IC50 value of 27.0 μg/ml when compared with standard vitamin E. The reducing power of the extract depends on the amount of extract. A significant amount of polyphenols could be detected by the equivalent to 0.0589 μg of pyrocatechol from 1 mg of extract.

Conclusion

The results obtained in the present study indicate that hydro alcoholic extract of aerial part of Cytisus scoparius is a potential source of natural antioxidants.