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Open Access Research article

Kaliziri extract upregulates tyrosinase, TRP-1, TRP-2 and MITF expression in murine B16 melanoma cells

Adila Tuerxuntayi124, Yong-qiang Liu12, Ablajan Tulake124, Maidina Kabas12, Aiden Eblimit3 and Haji Akber Aisa12*

Author Affiliations

1 The Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China

2 State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Urumqi 830011, China

3 Human Genome Sequencing Center Department of Molecular and Human Genetics Baylor College of Medicine, Houston, TX 77030, USA

4 University of the Chinese Academy of Sciences, Beijing 100039, China

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BMC Complementary and Alternative Medicine 2014, 14:166  doi:10.1186/1472-6882-14-166

Published: 22 May 2014

Abstract

Background

Kaliziri extract (KZE) is a traditional Uyghur medicine (TUM), used by traditional hospitals in China as an injection for treatment of vitiligo for more than 30 years. Clinical application has shown that this medicine has obvious therapeutic effects. However, its phytochemical analysis and mechanism have not been examined.

Methods

KZE was extracted from seeds of Kaliziri [Vernonia anthelmintica (L.) Willd.] in ethanol-water (80:20, v/v), its components were identified by LC-MS/MS, and the signaling pathway of melanin synthesis in KZE treated murine B16 melanoma cells was examined by western blotting.

Results

Liquid chromatography-mass spectrometry analysis confirmed that the main components of KZE are flavonoids. KZE increased the tyrosinase activity and melanin content in a dose-dependent manner at concentrations of 5-40 μg/ml, and treatment with 20 μg/ml of KZE enhanced the expression of tyrosinase in B16 cells in a time-dependent manner.

Conclusions

KZE induced melanogenesis by increasing the expression of TYR, TRP-1, TRP-2 and MITF in B16 cells.

Keywords:
KZE; Melanogenesis; B16 melanoma cells; TYR; MITF