FLP inhibits A549 cells invasion and inhibits pro-inflammatory cytokines when co-cultured with THP-1 macrophages. Effect of FLP on A549 cell invasion and pro-inflammatory cytokines TNF-α, IL-1β and IL-6. (A) Representative photomicrographs of A549 cells in matrigel invasion assay treated with NRS (Vehicle control), FLP (low, medium high) or CTX, after 48 hours. (B) Image Pro Plus Version 6.0 software was used to calculate migrated cells for each treatment, 5 fields were randomly chosen to average migrated cells, all the experiments were repeated 3 times. *P < 0.05 vs. NRS group. Measurements for pro-inflammatory cytokines were measure by ELISA data displayed as concentration of (C) TNF-α, (D) IL-1β and (E) IL-6 in the supernatant. *P < 0.05 vs. A549 + NRS group.
Li et al. BMC Complementary and Alternative Medicine 2014 14:153 doi:10.1186/1472-6882-14-153