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Open Access Research article

Memory-enhancing activities of the aqueous extract of Albizia adianthifolia leaves in the 6-hydroxydopamine-lesion rodent model of Parkinson’s disease

Galba Jean Beppe13*, Alain Bertrand Dongmo2, Harquin Simplice Foyet3, Nolé Tsabang6, Zenovia Olteanu4, Oana Cioanca5, Monica Hancianu5, Théophile Dimo1* and Lucian Hritcu4*

Author Affiliations

1 Laboratory of Animal Physiology, University of Yaoundé I, Yaounde, Cameroon

2 Department of Animal Biology and Physiology, University of Douala, Douala, Cameroon

3 Department of Agriculture, Cattle farming and Derived products, High Institute of the Sahel, University of Maroua, Maroua, Cameroon

4 Department of Biology, Alexandru Ioan Cuza University, Iasi, Romania

5 Faculty of Pharmacy, University of Medicine and Pharmacy “Gr. T. Popa”, Iasi, Romania

6 Centre for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plant Studies, Yaoundé, Cameroon

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BMC Complementary and Alternative Medicine 2014, 14:142  doi:10.1186/1472-6882-14-142

Published: 30 April 2014

Abstract

Background

Albizia adianthifolia (Schumach.) W. Wright (Fabaceae) is a traditional herb largely used in the African traditional medicine as analgesic, purgative, anti-inflammatory, antioxidant, antimicrobial and memory-enhancer drug. This study was undertaken in order to evaluate the possible cognitive-enhancing and antioxidative effects of the aqueous extract of A. adianthifolia leaves in the 6-hydroxydopamine-lesion rodent model of Parkinson’s disease.

Methods

The effect of the aqueous extract of A. adianthifolia leaves (150 and 300 mg/kg, orally, daily, for 21 days) on spatial memory performance was assessed using Y-maze and radial arm-maze tasks, as animal models of spatial memory. Pergolide - induced rotational behavior test was employed to validate unilateral damage to dopamine nigrostriatal neurons. Also, in vitro antioxidant activity was assessed through the estimation of total flavonoid and total phenolic contents along with determination of free radical scavenging activity. Statistical analyses were performed using two-way analysis of variance (ANOVA). Significant differences were determined by Tukey’s post hoc test. F values for which p < 0.05 were regarded as statistically significant. Pearson’s correlation coefficient and regression analysis were used in order to evaluate the association between behavioral parameters and net rotations in rotational behavior test.

Results

The 6-OHDA-treated rats exhibited the following: decrease of spontaneous alternations percentage within Y-maze task and increase of working memory errors and reference memory errors within radial arm maze task. Administration of the aqueous extract of A. adianthifolia leaves significantly improved these parameters, suggesting positive effects on spatial memory formation. Also, the aqueous extract of A. adianthifolia leaves showed potent in vitro antioxidant activity. Furthermore, in vivo evaluation, the aqueous extract of A. adianthifolia leaves attenuated the contralateral rotational asymmetry observed by pergolide challenge in 6-OHDA-treated rats.

Conclusions

Taken together, our results suggest that the aqueous extract of A. adianthifolia leaves possesses antioxidant potential and might provide an opportunity for management neurological abnormalities in Parkinson’s disease conditions.

Keywords:
Albizia adianthifolia extract; Antioxidant activity; 6-hydroxydopamine-lesion rat; Memory; Parkinson disease