Rac activity in resveratrol treated tumors. GFP-MDA-MB-231 or GFP-MDA-MB-435 mammary tumors from mice that received vehicle or 0.5, 5, or 50 mg/kg BW Res diets were lysed. PAK–PBD–GST beads were used to pull down active (GTP-bound) Rac from tumor lysates. Active and total Rac levels were detected by western blot analysis with anti-Rac antibody. Rac activity (active Rac/total Rac) relative to vehicle control as quantified from densitometric scans of western blot analyses is presented for the study performed with (A and B), MDA-MB-231 and (C-D), MDA-MB-435 cells. (A) and (C), Representative western blots for active (GTP-bound) Rac in the upper row and total tumoral Rac in the lower row for the MDA-MB-231 and the MDA-MB-435 studies, respectively. (B) and (D), Average relative Rac activity for the MDA-MB-231 and the MDA-MB-435 studies, respectively. Error bars represent SEMs from 2-4 tumors per group. Differences between means were determined using Student’s t-Test. Asterisk denotes statistical significance at p≤0.05.
Castillo-Pichardo et al. BMC Complementary and Alternative Medicine 2013 13:6 doi:10.1186/1472-6882-13-6