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Open Access Research article

Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats

Siti Syariah Mamat1, Mohamad Fauzi Fahmi Kamarolzaman1, Farhana Yahya1, Nur Diyana Mahmood1, Muhammad Syahmi Shahril1, Krystal Feredoline Jakius1, Norhafizah Mohtarrudin2, Siew Mooi Ching3, Deny Susanti4, Muhammad Taher5 and Zainul Amiruddin Zakaria1*

Author Affiliations

1 Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

2 Department of Pathology, Faculty of Medicine& Health Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

3 Department of Family Medicine, Faculty of Medicine and Health Sciences, 43400 UPM Serdang, Selangor, Malaysia

4 Department of Biomedical Science, Kulliyyah of Science, International Islamic University Malaysia, Jl Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang, Malaysia

5 Department of Pharmaceutical Technology, Kulliyyah of Pharmacy, International Islamic University Malaysia, Jalan Istana, Bandar Indera Mahkota, 25200 Kuantan, Pahang, Malaysia

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BMC Complementary and Alternative Medicine 2013, 13:326  doi:10.1186/1472-6882-13-326

Published: 23 November 2013

Abstract

Background

Melastoma malabathricum L. (Melastomaceae) is a small shrub with various medicinal uses. The present study was carried out to determine the hepatoprotective activity of methanol extract of M. malabathricum leaves (MEMM) against the paracetamol-induced liver toxicity in rats model.

Methods

The respective chemicals and herbal solutions (10% DMSO, 200 mg/kg silymarin or MEMM (50, 250 and 500 mg/kg)) were administered orally to rats once everyday for 7 days followed by the hepatotoxicity assay. The blood samples and livers were collected and subjected to biochemical and microscopical analysis. Prior to the hepatoprotective study, MEMM was subjected to determination of the total phenolic content (TPC) and the antioxidant properties using several standard assays (e.g. 2, 2-diphenyl-1-picrylhydrazyl- and superoxide anion- radical scavenging assay, and oxygen radical absorbance capacity assay).

Results

MEMM exerted significant (pā€‰<ā€‰0.05) and high antioxidant activity in which high TPC was recorded; while in the hepatotoxicity study, the extract exhibited significant hepatoprotective effects against the paracetamol-induced hepatotoxic model. The results observed for serum liver enzymes (ALT, ALP and AST) as well as the microscopic observations and microscopic scoring supported the hepatoprotective potential of MEMM. The phytochemical and HPLC analysis of MEMM demonstrated the presence of flavonoids as its major constituents.

Conclusions

The MEMM-induced hepatoprotective activity could be allied partly to its antioxidant activity and the presence of flavonoids.

Keywords:
Melastoma malabathricum; Melastomaceae; Methanol extract; Hepatoprotective activity; Antioxidant activity; Flavonoids