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Open Access Research article

In vitro and in vivo safety evaluation of Dipteryx alata Vogel extract

Natália Mencacci Esteves-Pedro1, Thaisa Borim1, Virginia Sbrugnera Nazato1, Magali Glauzer Silva1, Patricia Santos Lopes2, Márcio Galdino dos Santos3, Cháriston André Dal Belo4, Cássia Regina Primila Cardoso5, Eliana Aparecida Varanda5*, Francisco Carlos Groppo6, Marli Gerenutti1 and Yoko Oshima-Franco1

Author Affiliations

1 School of Pharmacy, University of Sorocaba, UNISO, Rodovia Raposo Tavares km 92.5, Zip code: 18023-000 Sorocaba, SP, Brazil

2 Federal University of São Paulo, UNIFESP, R. Prof. Artur Riedel, 275, Zip code: 09972-270 Diadema, SP, Brazil

3 Post-Graduation Course in Environmental Sciences, PGCiamb, Federal University of Tocantins, UFT, Av. NS 15 ALC NO 14, 109 Norte, Zip code: 77001-090 Tocantins, Brazil

4 Federal University of Pampa, UNIPAMPA, Av. Antonio Trilha, 1847, Zip code: 97300-000 São Gabriel, RS, Brazil

5 UNESP-São Paulo State University, Faculty of Pharmaceutical Sciences of Araraquara,Department of Biological Sciences, Rodovia Araraquara-Jaú, Km 1, 14801-902 Araraquara, São Paulo, Brazil

6 Piracicaba Dental School - State University of Campinas (UNICAMP), Av. Limeira 901, Zip code: 13414-903 Piracicaba, SP, Brazil

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BMC Complementary and Alternative Medicine 2012, 12:9  doi:10.1186/1472-6882-12-9

Published: 3 February 2012

Abstract

Background

Dipteryx alata Vogel popularly known as "baru" is an important commercial leguminous tree species from the Brazilian Cerrado, which possess medicinal properties, besides its fruits consumption by animals and humans. The use of the "naturally occurring plants" as herbal remedies and foods mainly from leaves, seeds, flowers and roots of plants or extracts require precautions before ensuring these are safe and efficacious. The objective of this study was to evaluate the safety of D. alata barks extract.

Methods

Vegetal drugs of D. alata barks were submitted to quality control assays and further to the safety assays under 1) in vitro parameter by Salmonella (Ames) mutagenicity, and 2) in vivo parameter on the pregnancy of rats.

Results

The extract was non-mutagenic to any of the assessed strains TA97a, TA98, TA100 and TA102 even after metabolic activation (+S9). All in vivo parameters (reproductive ability evaluation, physical development of rat offsprings, and neurobehavioral development assays) showed no changes related to control group.

Conclusion

D. alata barks extract is neither mutagenic by the Ames test nor toxic in the pregnancy of rats, with no physical-neurobehavioral consequences on the rat offsprings development.