Open Access Highly Accessed Research article

Chemical composition of 8 eucalyptus species' essential oils and the evaluation of their antibacterial, antifungal and antiviral activities

Ameur Elaissi1*, Zyed Rouis2, Nabil Abid Ben Salem2, Samia Mabrouk5, Youssef ben Salem3, Karima Bel Haj Salah2, Mahjoub Aouni2, Farhat Farhat4, Rachid Chemli1, Fethia Harzallah-Skhiri5 and Mohamed Larbi Khouja6

Author affiliations

1 Laboratory of Pharmacognosy, Faculty of Pharmacy, University of Monastir, Avenue Avicenne, Monastir, 5019, Tunisia

2 Laboratory of Transmissible Diseases and Biologically Active Substances, Faculty of Pharmacy, University of Monastir, rue Avicenne, Monastir, 5000, Tunisia

3 Laboratoire of Microbilogy, Imuunology, EPS Farhat Hachad, Sousse, Tunisia

4 Laboratory of Analytical Chemistry, Faculty of Pharmacy, University of Monastir, Avenue Avicenne, Monastir, 5019, Tunisia

5 Laboratory of Genetic, Biodiversity and Bio-resources Valorisation, Higher Institute of Biotechnology of Monastir, University of Monastir, Avenue Tahar Haddad, Monastir, 5000, Tunisia

6 National Institute for Research on Rural Engineering, Water and Forestry, Institution of Agricultural Research and Higher Education, Ariana, BP, N.2, 2080, Tunisia

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Citation and License

BMC Complementary and Alternative Medicine 2012, 12:81  doi:10.1186/1472-6882-12-81

Published: 28 June 2012



In 1957, Tunisia introduced 117 species of Eucalyptus; they have been used as fire wood, for the production of mine wood and to fight erosion. Actually, Eucalyptus essential oil is traditionally used to treat respiratory tract disorders such as pharyngitis, bronchitis, and sinusitis. A few investigations were reported on the biological activities of Eucalyptus oils worldwide. In Tunisia, our previous works conducted in 2010 and 2011 had been the first reports to study the antibacterial activities against reference strains. At that time it was not possible to evaluate their antimicrobial activities against clinical bacterial strains and other pathogens such as virus and fungi.


The essential oils of eight Eucalyptus species harvested from the Jbel Abderrahman, Korbous (North East Tunisia) and Souinet arboreta (North of Tunisia) were evaluated for their antimicrobial activities by disc diffusion and microbroth dilution methods against seven bacterial isolates: Haemophilus influenzae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus pneumoniae and Streptococcus pyogenes. In addition, the bactericidal, fungicidal and the antiviral activities of the tested oils were carried out.


Twenty five components were identified by GC/FID and GC/MS. These components were used to correlate with the biological activities of the tested oils. The chemical principal component analysis identified three groups, each of them constituted a chemotype. According to the values of zone diameter and percentage of the inhibition (zdi, % I, respectively), four groups and subgroups of bacterial strains and three groups of fungal strains were characterized by their sensitivity levels to Eucalyptus oils. The cytotoxic effect and the antiviral activity varied significantly within Eucalyptus species oils.


E. odorata showed the strongest activity against S. aureus, H. influenzae, S. agalactiae, S. pyogenes, S. pneumoniae and against all the tested fungal strains. In addition, E. odorata oil showed the most cytotoxic effect. However, the best antiviral activity appeared with E. bicostata. Virus pretreatment with E. bicostata essential oil showed better antiviral activity (IC50 = 0.7 mg/ml, SI = 22.8) than cell-pretreatment (IC50 = 4.8 mg/ml, SI = 3.33). The essential oil of E. astringens showed antiviral activity only when incubated with virus prior to cell infection. This activity was dose-dependent and the antiviral activity diminished with the decreasing essential oil concentration.

Eucalyptus sp.; Essential oil; Principal Components Analysis; Hierarchical Cluster Analysis; Antibacterial activity; Antifungal activity; Antiviral activity