Effects of isochlorogenic acid on NF-κB activity. (A) Isochlorogenic acid induces phosphorylation of IκBα. Cells were treated with isochlorogenic acid (500 μg/ml) for the indicated time periods. Lysates were subjected to immunoblotting. (B) NF-κB1- and NF-κB2-dependent binding of nuclear proteins in RAW264.7 cells. The cells were treated with isochlorogenic acid (500 μg/ml) for the indicated time periods before preparing nuclear extracts for analysis by EMSA with probe miNOS κB1 or κB2.
Tomimori et al. BMC Complementary and Alternative Medicine 2012 12:78 doi:10.1186/1472-6882-12-78