Effect of M. grandiflora L. flower extract on melanogenesis-related proteins expression. (A) B16F10 cells were cultured with α-MSH (100 nM) for 24 h and then treated with various concentration of the flower extract (10, 15, 20%; v/v) or kojic acid (200 μM) for another 24 hr. Then the content of cellular MITF, tyrosinase, TRP-1 and TRP-2 proteins were analyzed by western blotting assay. (B) The relative amounts of MITF, tyrosinase, TRP-1 and TRP-2 compared to total GAPDH were calculated and analyzed by Multi Gauge 3.0 software and the values represented the mean of triplicate experiments ± S.D.
Huang et al. BMC Complementary and Alternative Medicine 2012 12:72 doi:10.1186/1472-6882-12-72