Effects of Huangqi decoction (HQD) on histological changes (A, Sirius Red staining original magnification × 100; B, α-SMA staining original magnification × 100), C, hydroxyproline content, and D, the expression of pro-fibrotic factors of rat livers in DMN-induced liver fibrosis. ▴ p < 0.05, ▴▴p < 0.01, vs. DMN-water rats; △p < 0.05, △△p < 0.01 vs. 2 weeks DMN rats. Western blot analysis of total protein extracts was performed by antibodies recognizing α-SMA, TIMP-1, TGF-β1 and GAPDH respectively. Results are mean ± SD.
Liu et al. BMC Complementary and Alternative Medicine 2012 12:51 doi:10.1186/1472-6882-12-51