Representative TLC plates showing the compound profile. (A), Lanes 1–8 contain fractions 31, 33, 35, 37, 39, 41, 43 and 45, respectively, from the Sephadex LH-20 chromatography of CDEb5-5. The potentially pure band of fractions# 33–45 (pooled as compound I) is indicated by an arrow. (B), Lane 1 contains CDEb5 while lane 2 contains pooled fractions# 70–109 from CDEb5–6 (compound II, indicated by an arrow). The solvent system used in the shown TLC plates were (A) 1:19 (v/v) methanol: DCM and (B) 1:9 (v/v) methanol: DCM. TLC plates shown are representative of three replicates.
Chantarudee et al. BMC Complementary and Alternative Medicine 2012 12:45 doi:10.1186/1472-6882-12-45