Table 3

The yield and character of the five CHE fractions obtained after quick column chromatography

Fraction

Weight (mg)

Yield (% of

CHE/total propolis)

Character

Antiproliferative/

cytotoxic activitya

TLC platesb


I

80

0.36%/0.09%

Clear wax

--

3


II

20

0.09%/0.02%

Clear yellow oil

--

3


III

320

1.42%/0.36%

Yellow oil

3 cell lines

1


IV

270

1.2%/0.30%

Yellow powder

2 cell lines

3


V

4,300

19.1%/4.78%

Dark brown oil

all 5 cell lines

2


aNumber of the five human cancer cell lines in which a significant antiproliferative/cytotoxic activity was induced by the extract after a 72 h exposure, as determined by the MTT assay

bThe minimum number of distinct compounds in the fraction as determined by evaluation of the different TLC plates

Teerasripreecha et al. BMC Complementary and Alternative Medicine 2012 12:27   doi:10.1186/1472-6882-12-27

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