Fluorescence microscopy of HeLa cells treated with crude aqueous root extracts of P. indica for 48 hours and double stained with annexin-V-sensitive probe (conjugated to FITC) and propidium iodide. Qualitative labeling of annexin V in the plasma membrane (B and E) or cellular uptake of propidium iodide (C and F) was recorded. Control cells exposed to PBS (A)-(C), and 0.5 mg/ml of crude aqueous extracts treated cells (D)-(F) were photographed with a microscope (× 40).
Cho et al. BMC Complementary and Alternative Medicine 2012 12:265 doi:10.1186/1472-6882-12-265