Effects of SCTE on MMP-9 activity and protein expression in lung tissues of mice. The protein was loaded for gelatin zymography (60 μg/lane). SDS-PAGE zymography was performed according to previous method . (A) MMP-9 activity and protein expression. (B) Densitometry of MMP-9. PBS/PBS, PBS-sensitized/challenged, negative control (PBS only); OVA/PBS, OVA-sensitized/challenged mice (PBS only); OVA/mon, OVA-sensitized/challenged mice (montelukast 30 mg/kg); OVA/SCTE-200, OVA-sensitized/challenged mice (SCTE 200 mg/kg); OVA/SCTE-400, OVA-sensitized/challenged mice (SCTE 400 mg/kg). SCTE or montelukast was given 1 h before the challenge. Significantly different from PBS/PBS, #P < 0.05, ##P < 0.01; significantly different from OVA, *P < 0.05, **P < 0.01.
Lee et al. BMC Complementary and Alternative Medicine 2012 12:257 doi:10.1186/1472-6882-12-257