Frankincense essential oil-induced caspase apoptotic pathway in pancreatic cancer cells. An aliquot of 5 × 105 pancreatic cancer MIA PaCa-2 cells were seeded in each of 60 mm tissue culture plates. Adherent cells were then subjected to Fractions III (1:600 dilution) or IV (1:1,200 dilution) essential oil stimulation. Total cellular proteins were isolated between 0 (untreated control) and 4 h after treatment. Levels of pro- and activated-caspase-8, 9, 3 and PARP in MIA PaCa-2 cells were detected by Western blot analysis before and after frankincense essential oil treatment. Levels of β-actin was determined in the same samples and used as a protein loading control.
Ni et al. BMC Complementary and Alternative Medicine 2012 12:253 doi:10.1186/1472-6882-12-253