Quantitative analysis of frankincense essential oil-induced pancreatic cancer cell death. Human pancreatic cancer cells were seeded into each well of 96-well tissue culture plates at the concentration of 5 × 103/100 μl. After adherence, cells were subjected to serial dilutions of either (A) Fraction III or (B) Fraction IV essential oil treatment. At 3 h post-treatment, cell viability was determined by the LDH cytotoxicity detection kit. Experiments were prepared in triplicate; and results are presented as mean % of cell death ± SEM in relation to untreated cells from at least 3 independent experiments. * indicates statistical difference of cell death between essential oil-treated and untreated cells (P < 0.05).
Ni et al. BMC Complementary and Alternative Medicine 2012 12:253 doi:10.1186/1472-6882-12-253