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Open Access Open Badges Research article

Inhibition of lipopolysaccharide-induced nitric oxide and prostaglandin E2 production by chloroform fraction of Cudrania tricuspidata in RAW 264.7 macrophages

Gabsik Yang, Kyungjin Lee, Mihwa Lee, Inhye Ham and Ho-Young Choi*

Author Affiliations

Department of Herbology, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Seoul, Dongdaemun-Gu, Republic of Korea

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BMC Complementary and Alternative Medicine 2012, 12:250  doi:10.1186/1472-6882-12-250

Published: 10 December 2012



Cudrania tricuspidata extract is an important traditional herbal remedy for tumors, inflammation, gastritis, and liver damage and is predominantly used in Korea, China, and Japan. However, the anti-inflammatory effects of the extract have not yet been conclusively proved.


In this study, we investigated the effects of the CHCl3 fraction (CTC) of a methanol extract of C. tricuspidata on the lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production in RAW 264.7 macrophage cells and mouse peritoneal macrophages, and the levels of pro-inflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 in RAW 264.7 macrophage cells.


We observed that the protein expression levels of inducible NO synthase and COX-2 enzymes were markedly inhibited by CTC in a concentration-dependent manner. In addition, CTC reduced the production of TNF-α, IL-1β, and IL-6 in the LPS-stimulated RAW 264.7 macrophage cells.


Our results show that the C. tricuspidata extract could modulate macrophage-mediated inflammatory functions such as the overproduction of cytokines, NO, and PGE2. The CTC was found to be the active fraction in this context.

Cudrania tricuspidata; Nitric oxide; Prostaglandin E2; Lipopolysaccharide CHCl3 partitioned methanol extract of Cudrania tricuspidata; CTC, Lipopolysaccharide; LPS, prostaglandin E2; PGE2, nitric oxide; NO