The protection of DG against hypoxia and reoxygenation (H/R)-induced injury in H9c2 cells. After 12 h of hypoxia, the solution was changed into a normal medium with or without DG (125 and 250 μg/ml) or trolox (20 μM) in the 4 hours reoxygenation period. Cell viability was then measured by MTT assay. The data were expressed as mean ± S.E.M. Six replicates were done in each experiment. Three independent experiments were performed. *p < 0.05, **p < 0.01 and ***p < 0.001 indicated the groups with significant difference when compared to the control group; ### p < 0.001 when compared to the normoxic group.
Hu et al. BMC Complementary and Alternative Medicine 2012 12:249 doi:10.1186/1472-6882-12-249