Figure 3.

Effects of crude extracts on the level of cccDNA in transiently transfected HepG2 cells with the DNA expression plasmid of HBV genome. HepG2 cells were treated with either 30 μg/mL of hydroalcoholic extracts or 0.3 μg of buffer extracts and were transfected with 1 μg of the DNA expression plasmid of HBV genome using Lipofectamine ™ 2000 in triplicate. After 5 days of incubation, the total DNA of each well was extracted and subjected for quantitative real-time analysis. Bar graph represents a mean value whereas an error bar indicates the uncertainty value of three independent experiments. “*” and “**” indicate significant inhibitory effect when compared with the positive cccDNA at p < 0.01 and p < 0.001 (by t- test) respectively. “F” is for fruit and “L” is for leaf.

Waiyaput et al. BMC Complementary and Alternative Medicine 2012 12:246   doi:10.1186/1472-6882-12-246
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