Effects of crude extracts on the level of cccDNA in transiently transfected HepG2 cells with the DNA expression plasmid of HBV genome. HepG2 cells were treated with either 30 μg/mL of hydroalcoholic extracts or 0.3 μg of buffer extracts and were transfected with 1 μg of the DNA expression plasmid of HBV genome using Lipofectamine ™ 2000 in triplicate. After 5 days of incubation, the total DNA of each well was extracted and subjected for quantitative real-time analysis. Bar graph represents a mean value whereas an error bar indicates the uncertainty value of three independent experiments. “*” and “**” indicate significant inhibitory effect when compared with the positive cccDNA at p < 0.01 and p < 0.001 (by t- test) respectively. “F” is for fruit and “L” is for leaf.
Waiyaput et al. BMC Complementary and Alternative Medicine 2012 12:246 doi:10.1186/1472-6882-12-246