UV spectrum overlay of Indirubin standard and the corresponding peak of chloroform extract of the fruit of Couroupita guianensis. The retention time of the standard compound with UV detection at 254 nm was about 13.4 min. The UV spectral similarity was also confirmed by the overlay of the UV spectrum of the standard to the corresponding peak of chloroform extract of the fruit of C. guianensis, extracted from PDA detection.
Al-Dhabi et al. BMC Complementary and Alternative Medicine 2012 12:242 doi:10.1186/1472-6882-12-242