In vitro effect of CWE on LPS-induced IκBα degradation and MAP kinase activation. Peritoneal macrophages were pretreated with the indicated concentration of CWE for 1 h and then stimulated with LPS for 15 min. Phospho-IKK, IκBα, phospho-IκBα,phospho-JNK, JNK, phospho-p38, p38, phospho-ERK1/2, and ERK1/2 in whole protein extracts were examined by Western blot analysis. Tubulin was used as an internal control. One of the five experiments is shown.
Hong et al. BMC Complementary and Alternative Medicine 2012 12:237 doi:10.1186/1472-6882-12-237