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Open Access Research article

Evaluation of anxiolytic activity of compound Valeriana jatamansi Jones in mice

Jie-Shu You1, Min Peng1, Jin-Li Shi1*, Hu-Zhan Zheng1, Yong Liu1, Bao-Sheng Zhao1 and Jian-You Guo2*

Author affiliations

1 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, P.R. China

2 Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, P.R. China

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Citation and License

BMC Complementary and Alternative Medicine 2012, 12:223  doi:10.1186/1472-6882-12-223

Published: 21 November 2012

Abstract

Background

Compound Valeriana jatamansi Jones is a formula for treating anxiety-related diseases in the clinic, which is composed of Valeriana jatamansi Rhizoma et Radix, Ziziphi Spinosae Semen, Albiziae Cortex and Junci Medulla. The purpose of this study was to explore the anxiolytic properties of this compound in mice.

Methods

Male ICR mice were treated with compound Valerianae Jatamansi Jones (1.2 g/kg, 2.4 g/kg, 4.8 g/kg), saline, diazepam (2 mg/kg) orally for 10 days and then exposed to elevated maze-plus (EPM) and light–dark box (LDB). The effects of the compound on spontaneous activity were evaluated by locomotor activity test. We further investigated the mechanism of action underlying the anxiolytic-like effect of compound by pre-treating animals with antagonists of benzodiazepine (flumazenil, 3mg/kg) prior to evaluation using EPM and LDB.

Results

Compound Valerianae Jatamansi Jones (2.4, 4.8 g/kg, p.o.) significantly increased entries (P<0.05) into and time spent (P<0.05) on the open arms of the EPM, and number of transitions (P<0.05) and time spent (P<0.05) in the light compartment of the LDB. However, the anxiolytic-like effects of compound were significantly reduced by pre-treatment with flumazenil (P>0.05). In addition, compound Valerianae Jatamansi Jones treatment didn’t affect the spontaneous activity in mice (P> 0.05).

Conclusions

The present study supports the hypothesis that compound Valeriana jatamansi Jones exert anxiolytic action but no sedative effects in mice and that this effect might be mediated by benzodiazepine receptors.

Keywords:
Compound Valeriana jatamansi Jones; Anxiolytic; Elevated maze-plus; Light–dark box; Benzodiazepine receptors