The effects of the leaf extracts of P. betle on the proliferation of MCF-7 cells. Cells were grown in RPMI 1640 medium supplemented with 10% (v/v) FBS, 10 μg/ml BSA and antibiotics, at 37°C in a humidified atmosphere containing 5% CO2. Confluent cells (5 X 103 cells/well) were treated with the extracts of P. betle (25–200 μg/ml) for 48 h and cell viability was determined using the MTT assay.
Abrahim et al. BMC Complementary and Alternative Medicine 2012 12:220 doi:10.1186/1472-6882-12-220