Table 2

Revertants/ plate, standard deviation and mutagenicity index (in brackets) for the strains TA98, TA100, TA102 and TA97a of S. typhimurium after treatment with various doses of HK, with (+S9) and without (−S9) metabolic activation
Treatments Number of revertants (M±SD)/ plate and MI
μg/plate TA 98 TA 100 TA 102 TA 97a
- S9 + S9 - S9 + S9 - S9 + S9 - S9 + S9
HK 0.0a 22 ± 2 32 ± 1 132 ± 6 123 ± 1 243 ± 4 391 ± 8 164 ± 4 163 ± 2
9.7 18 ± 4 (0.8) 32 ± 1 (1.0) 134 ± 4 (1.0) 130 ± 6 (1.1) 238 ± 8 (1.0) 376 ± 7 (1.0) 165 ± 5 (1.0) 172 ± 10 (1.0)
19.5 23 ± 3 (1.0) 34 ± 3 (1.1) 129 ± 6 (1.0) 128 ± 6 (1.0) 231 ± 8 (0.9) 363 ± 3 (0.9) 165 ± 9 (0.9) 187 ± 3 (1.1)
39.0 25 ± 3 (1.1) 33 ± 4 (1.0) 134 ± 5 (1.0) 115 ± 8 (0.9) 213 ± 4 (0.9) 354 ± 4 (0.9) 146 ± 5 (0.9) 166 ± 5 (1.0)
58.5 25 ± 2 (1.1) 28 ± 1 (0.8) 112 ± 3 (0.8) 134 ± 2 (1.1) 201 ± 6 (0.8) 398 ± 9 (1.0) 175 ± 4 (1.1) 172 ± 2 (1.0)
78.0 24 ± 5 (1.1) 31 ± 2 (1.0) 140 ± 9 (1.1) 120 ± 8 (1.0) 186 ± 2 (0.8) 402 ± 3 (1.0) 163 ± 8 (1.0) 146 ± 4 (0.9)
Ctrol + 1347 ± 88b 1567 ± 115e 1582 ± 98c 1456 ± 78e 1656 ± 60d 1932 ± 97f 1766 ± 49b 1789 ± 89e

HK = (−)-Hinokinin; M ± SD = mean and standard deviation; MI = mutagenicity index; aNegative control: dimethylsulfoxide (DMSO - 50 μL/ plate); Ctrol + = Positive control - b4 -nitro-o-phenylenediamine (NOPD – 10.0 μg/ plate – TA98, TA97a); csodium azide (1.25 μg/ plate – TA100); dmitomycin (0.5 μg/ plate – TA102), in the absence of S9 and e2-anthramine (1.25 μg/ plate – TA 97a, TA98, TA100); f2-aminofluorene (10.0 μg/ plate – TA102), in the presence of S9.

Resende et al.

Resende et al. BMC Complementary and Alternative Medicine 2012 12:203   doi:10.1186/1472-6882-12-203

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