mRNA expressions of stemness genes and EMT markers analyzed by q-RT-PCR. The amounts of Sox-2, Oct-4, Klf-4 and EMT markers Vimetin, Twist, Snail and N-cad were increased in TW01 sphere-derived cells as compared to parental cells. The internal control is housekeeping gene-GAPDH and every gene expression of sphere-derived cells was normalized with parental cells. Data represent mean ± SD. * means significantly different from respective controls, p < 0.05.
Lin et al. BMC Complementary and Alternative Medicine 2012 12:201 doi:10.1186/1472-6882-12-201