Additional file 1.
Extraction and isolation of cucurbitacin B. The dried fruit fibers of T. cucumerina L. (2.72 kg) were chilled in liquid N2, milled to small pieces and extracted successively with n-hexane, EtOAc and MeOH in a Soxhlet extraction apparatus. The extracts were evaporated to dryness under reduced pressure at temperature 40-45°C. The hexane extract (greenish viscous oil, 14.9 g), the EtOAc extract (greenish sticky solid, 83.2 g) and the MeOH extract (dark brownish amorphous, 257.8 g) were respectively obtained. The extraction sequence is shown in Figure 1.
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Duangmano et al. BMC Complementary and Alternative Medicine 2012 12:185 doi:10.1186/1472-6882-12-185