Effect of cucurbitacin B on cell-cycle distribution. MCF-7 and MDA-MB-231 were treated with 0, 2.5, and 5 μM cucurbitacin B for 24 hr, and then stained with propidium iodide (PI) before subjected to flow cytometric analysis. A. The cell cycle/DNA content histograms represent the cell population at each cell cycle phase as determined by the level of DNA content in each cucurbitacin B treatmented group. Blockage at G2/M and apoptotic induction was observed. B. The values indicate percentage of cells in each phases of the cell cycle. *, p<0.05 (treated vs untreated control).
Duangmano et al. BMC Complementary and Alternative Medicine 2012 12:185 doi:10.1186/1472-6882-12-185