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Cucurbitacin B inhibits human breast cancer cell proliferation through disruption of microtubule polymerization and nucleophosmin/B23 translocation

Suwit Duangmano134, Phorntip Sae-lim2, Apichart Suksamrarn2, Frederick E Domann3 and Pimpicha Patmasiriwat4*

Author Affiliations

1 School of Allied Health Science and Public Health, Walailak University, Bangkok, Thailand

2 Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Ramkhamhaeng University, Bangkok, Thailand

3 Free Radical and Radiation Biology Program, Department of Radiation Oncology, University of Iowa, Iowa City, IA, 52242, USA

4 Faculty of Medical Technology, Mahidol University, Bangkok, Thailand

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BMC Complementary and Alternative Medicine 2012, 12:185  doi:10.1186/1472-6882-12-185

Published: 12 October 2012

Additional files

Additional file 1:

Extraction and isolation of cucurbitacin B. The dried fruit fibers of T. cucumerina L. (2.72 kg) were chilled in liquid N2, milled to small pieces and extracted successively with n-hexane, EtOAc and MeOH in a Soxhlet extraction apparatus. The extracts were evaporated to dryness under reduced pressure at temperature 40-45°C. The hexane extract (greenish viscous oil, 14.9 g), the EtOAc extract (greenish sticky solid, 83.2 g) and the MeOH extract (dark brownish amorphous, 257.8 g) were respectively obtained. The extraction sequence is shown in Figure 1.

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Additional file 2:

Figure Protein expression of nucleophosmin/B23 (Nucl./B23), STAT3, tubulin, and c-Myc were determined by western blot analysis. MCF-7 and MDA-MB-231 were treated without or with cucurbitacin B for 48 hrs. After incubation, total proteins were extracted and performed western blotting to analyze the expression levels of nucleophosmin, STAT3, tubulin, and c-Myc gene. This bar graph represents the densitometric analyses of expression of nucleophosmin/B23 (Nucl./B23), STAT3, tubulin, and c-Myc relative to the untreated control. * P < 0.05 (treated vs untreated control).

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